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Fig. 6. Hedgehog signaling accelerates chondrocyte hypertrophy in postnatal
cartilage. (A) Skeletal preparations of P15 forelimb and hindlimb
of the Ptch1c/c;Col2a1-CreER and wild-type mouse.
Unmineralized cartilage in the hand, shoulder and knee joints and the scapula
was reduced in the mutant, as shown at high magnification. (Ba-d)
Serial sections of distal femur of P12 mice were stained with Safranin O and
used for immunohistochemistry to detect Col2a1 expression. There were more
hypertrophic chondrocytes with reduced Col2a1 expression in the
Ptch1c/c;Col2a1-CreER mouse. (Be-j) Sections of
proximal humeri from one-year-old mice were stained with Safranin O. Boxed
regions in e and f are shown at higher magnifications in g,h. Cartilage lining
was thinner in the Ptch1c/c;Col2a1-CreER mouse (arrow).
(i,j) Higher magnification of articular cartilage of the proximal humerus.
Joint cartilage in the Ptch1c/c;Col2a1-CreER mouse was
significantly reduced (double-headed arrows). (Ca-d) Sections of
proximal humeri from 14-month-old mice were stained with Safranin O. Boxed
regions in a and b are shown at higher magnifications in c,d. Cartilage lining
was thicker in the Smoc/c;Col2a1-CreER mouse (arrow).
(Ce,f) Higher magnification of articular cartilage of the
proximal humerus. Joint cartilage in the
Smoc/c;Col2a1-CreER mouse was significantly thicker
(double-headed arrows).
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