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Fig. 1. Absence of AChR clusters during initial stages (E13-E15.5) of
neuromuscular synaptogenesis in 
-/- embryos.
Clustering of AChRs was detected by Texas Red-conjugated 
-bungarotoxin.
In whole-mount diaphragm muscles (A-C,F-H), AChR clusters were
distributed along a central region of the muscle in the wild type (arrowheads
in A-C), but were absent from the -/- muscles (F-H);
instead, diffused fluorescence was observed on the surface of the muscles.
Broken lines in H indicate the central region of the muscle. Similarly, AChR
clusters were detected in EDL or soleus muscles in the wild-type
(D,E), but not in the -/- muscles
(I,J). Scale bars: 25 µm in A-C,F-H; 20 µm in D,E,I,J.
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