spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Increased nerve branching and broadening of the innervation band in {gamma}-/- muscles. Whole-mount diaphragm muscles were immunostained with antibodies against neurofilament (A,B), syntaxin (C,D), synaptophysin (E,F) or synaptotagmin 2 (Syt2) (G,H). (A,B) The hemi-diaphragm; (C-H) the dorsal quadrant diaphragm. In E13 wild-type muscle, collateral axons emanated from the nerve trunk in an orderly fashion and were mainly confined to the central region of the muscle (arrowheads in A); in E13 {gamma}-/- muscle, noticeable increases of axons were observed and distributed across a broad region of the muscle (B). Similarly, at E15.5, intramuscular nerves were confined to the central region of wild-type muscle (C,E,G), but projected aberrantly to a broad region of the {gamma}-/- muscles (D,F,H). Numerous puncta labeled by either synaptophysin or Syt2 antibodies at the nerve terminals are observed in both the wild-type and {gamma}-/- muscles (arrowheads in E-H, also see inset in G,H). Scale bar: 200 µm.





Right arrow Return to article