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Figure 8


Fig. 8. Localization of MuSK in the absence of the {gamma}-subunit. Whole-mount E15.5 diaphragm muscles from the ventral costal region (A-D) and sternal region (E-H) of wild-type (A-B,E-F) and {gamma}-/- (C-D,G-H) embryos were doubly labeled with MuSK antibody and {alpha}-bungarotoxin. In wild-type muscles, MuSK staining (arrowheads in B and F) was detected at the central region of the muscle, corresponding to AChR clusters labeled by {alpha}-bungarotoxin (arrowheads in A,E). In the {gamma}-/- muscles, MuSK staining was also seen at the central region of the muscle (arrows in D,H), despite the lack of AChR clustering (C,G). Scale bar: 10 µm.





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