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Fig. 2. wisp function in the Drosophila germline. (A-E) Meiotic arrest as visualized in embryos. Immunostaining of 0- to 20-minute wild-type embryos and 0- to 2-hour wisp^KG5287/Df(1)RA47 embryos with anti--tubulin (green) and DAPI (blue). (A) Wild-type embryo showing mitoses. (B) wisp^KG5287/Df(1)RA47 embryo showing one metaphase I anastral female spindle and one mitotic-like spindle with a centrosome, associated with the male pronucleus (arrow). (C,D) Examples of meiosis I spindle in wisp^KG5287/Df(1)RA47 embryos showing scattered chromosomes along the spindle (C), and asymmetric pools of chromosomes (D). Bottom panels are stained with DAPI alone. (E) Scoring of meiotic arrest as visualized with anti--tubulin and DAPI staining. (F-J) Meiotic defects in stage 14 oocytes visualized with anti--tubulin (green) and DAPI (blue). (F) Prometaphase I in wild-type stage 14 oocyte. (G) Wild-type-like metaphase I in wisp^KG5287/Df(1)RA47 embryo; the fourth chromosomes are separated from the main chromosome pool (metaphase I arrest in J). (H,I) Abnormal metaphase I spindles in wisp^KG5287/Df(1)RA47 oocytes. Bottom panels are stained with DAPI alone. (J) Scoring of meiotic figures as visualized with anti--tubulin and DAPI. (K,L) DAPI staining of stage 8 oocytes, showing that the karyosome is not affected in the wisp mutant. (K) Wild type; (L) wisp^KG5287/Df(1)RA47.

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