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Fig. 2. Direct activation of GL3 target genes by 35S::TTG1-GR. (A)
Gene expression levels were measured by relative quantitative PCR. The results
were calculated using the comparative Ct method (ABI bulletin) and presented
as fold changes compared with the mock or CHX treatment, which were
standardized to the level of actin expression. The induced expression levels
of GL2, TTG2, CPC and ETC1 were statistically significantly different from
those of control treatments (P<0.05); error bars indicate the
ranges of expression change; nt, not tested. (B) Semi-quantitative PCR
of ChIP experiments using ttg1/pTTG1::YFP-TTG1 (left) or
gl1/pGL1::GL1-YFP-cMYC (right). PCRs were performed on three fourfold
serial dilutions of the immunoprecipitated material, represented by the black
slope.
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