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Figure 6


Fig. 6. GL3 and EGL3 have overlapping but distinct expression patterns. (A-D) Transcription patterns of GL3 and EGL3 promoter::GUS in wild-type leaves. (A,C) At a very young stage, both GL3 and EGL3 are strongly transcribed in undifferentiated leaf primordia; in later differentiating leaves, high GL3 is observed in undifferentiated cells close to the basal edge of the leaf, while EGL3 is more widespread and not restricted to the edge; trichome initials show higher levels of GL3 and EGL3 than surrounding epidermal cells. (B,D) At a more mature stage, strong GL3 expression becomes restricted to trichomes, while EGL3 expression persists in pavement cells as well as in trichomes. (E-H) Protein accumulation patterns of GL3 and EGL3 in wild-type leaves. (E,F) GL3-YFP highly accumulates in trichome initials (arrowheads) and young trichomes. (G,H) At the same stages, strong EGL3-YFP was detected in the trichomes (arrowheads) and non-trichome cells. Scale bars: 20 µm in E,G; 50 µm in F,H.





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