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Fig. S1. The effects of Tlr2 and Tlr4 siRNA on Il6 mRNA expression. The effects of Tlr2 and/or Tlr4 siRNA transfection of granulosa cells on the expression of Il6 mRNA were compared with those of anti-TLR2 and/or anti-TLR4 neutralizing antibodies.
Fig. S2. The effects of CCL2, CCL4 or CCL5 on sperm motility.
Fig. S3. The COC-secreted factors induced sperm motility and capacitation. Sperm were added to one side of the chamber, and 10 µl of conditioned medium from COCs cultured with sperm for 12 hours was added to other.
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