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Figure 2


Fig. 2. HA fragments induced the TLR2-, TLR4- and CD44-targeted signal transduction pathways. (A) Time-dependent changes of the activation of p38MAP kinase, ERK1/2 and the NF{kappa}B pathway in cumulus cells of ovulated COCs cultured with 100 µg/ml of HA fragments. Results in each panel are representative of two separate experiments. (B) Ovulated COCs were pre-cultured with neutralizing antibodies (both anti-TLR2 and anti-TLR4, or anti-CD44) for 30 minutes, and further cultured with HA fragments for 15 minutes (p38MAP kinase and ERK1/2), or for 2 hours (I{kappa}β and NF{kappa}B). Results in each panel are representative of two separate experiments.





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