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Figure 3


Fig. 3. The effects of hyaluronidase (HY) treatment on gene expression and NF{kappa}B pathway activation in cumulus cells of ovulated COCs. (A) Dose-dependent effects of HY on the expression of cytokine and chemokine mRNA. Ovulated COCs were treated with 0 to 100 mIU/ml of HY for 2 hours. The 0 hour value was set as 1, and the data are presented as fold increase. Values are mean±s.e.m. of three replicates. Significant differences were observed as compared with COCs cultured without HY for 2 hours (Cont; *P<0.01; **P<0.05). (B) HY-induced cytokine and chemokine mRNA expression and NF{kappa}B pathway activation were suppressed by both anti-TLR2 and anti-TLR4 neutralizing antibodies (Ab). Ovulated COCs were pre-cultured with both anti-TLR2 and anti-TLR4 antibodies for 30 minutes, then 10 mIU/ml of HY was added to the medium. The addition of neutralizing antibodies significantly suppressed Il6, Ccl2, Ccl4 and Ccl5 gene expression (*P<0.01; **P<0.05). Cont, COCs cultured without HY for 2 hours. Results of western blotting in each panel are representative of two separate experiments.





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