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Fig. 4. Requirement of FoxM1-dependent G2-M progression for neuronal
differentiation. (A) Xenopus embryos were analyzed for
Cdc25B and cyclin B3 by RT-PCR. (B) Embryos pre-injected with
control MO or Cdc25B-MO (18 ng) at the one-cell stage were cultured until st.
32 and photographed to show morphological phenotypes. (C) Embryos
pre-injected with both lacZ mRNA (100 pg) and control MO or Cdc25B-MO
(18 ng) at one blastomere at the two-cell stage were fixed at st. 13,
immunostained for pH3, and analyzed as in
Fig. 1B.
*P<0.01. (D) Embryos were co-injected with
lacZ mRNA (100 pg) and either control MO or Cdc25B-MO (18 ng) at one
blastomere at the two-cell stage, cultured until st. 14, and analyzed by WISH.
In each panel, the injected side (β-Gal, red) of the embryo is on the
right (dorsal view, anterior up). (E) Animal caps from the late
blastula embryos pre-injected with control MO or Cdc25B-MO (36 ng) at the
one-cell stage were cultured with or without Activin (200 pM) until sibling
embryos reached st. 20 and were then analyzed by RT-PCR (left panel). Animal
caps pre-treated with Noggin mRNA (100 pg) and either control MO or Cdc25B-MO
(36 ng) were cultured and analyzed by RT-PCR (right panel). (F) Embryos
pre-injected with control MO or FoxM1-MO (36 ng) together with or without
Cdc25B mRNA (200 pg) at the one-cell stage were cultured until st. 32
and photographed (left panels). For RT-PCR analysis (right panel), embryos
were collected at st. 15.
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