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Fig. 8. Mash1 is expressed but not required in dentate granule progenitor generation. (A-C) Sections through the dentate gyrus of P1 wild-type mice immunolabelled for (A,A1) Ngn2 and Mash1, showing that many Mash1⁺ progenitors co-express Ngn2 (arrows), and for (B,B1) Mash1 and Prox1, showing that most Mash1⁺ cells co-express Prox1^low. (C,C1) Section through the dentate gyrus of P1 Mash1::GFP transgenic mice immunolabelled for Ngn2 and GFP shows that many Ngn2⁺ progenitors co-express GFP. (D,D1) Section through the dentate gyrus of P1 Ngn2^KIGFP/+ mice immunolabelled for Mash1 and GFP, show that many Mash1⁺ progenitors co-express GFP. (A1-D1) High magnifications of the areas outlined in A-D. Arrows indicate co-expression of markers and arrowheads indicate cells expressing only one marker. (E-F''') Sections through the dentate gyrus of E18.5 wild-type (E,F), Mash1 mutant (E',F'), Ngn2 mutant (F'') and Mash1; Ngn2 double mutant (F''') embryos showing the expression of Neurod1 transcripts by in situ hybridisation (E,E') and Prox1 protein (F-F'''). Mash1 mutant and wild-type embryos have similar patterns of Neurod1 (E,E') and Prox1 (F,F') expression. (G,H) Histograms representing the number of cells expressing Prox1^high (G) and Prox1^low (H) in the different genotypes shown in F-F'''. The defect in Prox1 expression is not more severe in the dentate gyrus of Mash1^/; Ngn2^KIGFP/GFP double mutants than in Ngn2^KIGFP/GFP single mutants. Scale bars: 20 µm in A-D; 50 µm in E-F'''.

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