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Fig. 9. Abnormal distribution of neuronal progenitors in Ngn2 mutant dentate gyrus. (A-D') Sections through the hippocampus of Ngn2^KIGFP/+ (A-D) or Ngn2^KIGFP/GFP (A'-D') mice at E18.5 (A,A'), P3 (B,B') and P5 (C,C',C1,C'1,D,D'), immunolabelled for GFP together with Neurod1 (A,A',D,D') or BrdU and GFAP (C,C',C1,C'1). (C1,C1') High magnification of the areas outlined in C,C' showing green/red and white channels separately. (C1,C'1) The radial glial scaffold labelled by GFAP is absent from the Ngn2^KIGFP/GFP DG. Note the lack of GFP-expressing cells at the position of the tertiary matrix in Ngn2^KIGFP/GFP mice. Square brackets indicate cells in an outer position. (E) Histogram representing the number of GFP⁺ cells in secondary, tertiary and outer positions at E18.5. (F) Histogram representing the percentage of Ngn2-expressing cells in outer positions of the wild-type forming DG, labelled with BrdU (30-minute pulse) or Prox1^low at E18.5. (G) Histogram representing the percentage of GFP-expressing cells labelled with BrdU (30 minutes), Neurod1, Prox1^low (light colour) or Prox1^high (dark colour) in Ngn2^KIGFP/+ (blue bars) and Ngn2^KIGFP/GFP (red bars) in outer positions of the dentate gyrus at E18.5. DG, dentate gyrus; 3ry, tertiary matrix; Out, outer position. Scale bars: 100 µm in A-C'; 20 µm in C1-D'.

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