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Fig. 3. Skeletal muscle defects in Tln1HSA-CREko mice. (A-P)
Muscle sections from wild-type and mutant animals were stained with
Hematoxylin and Eosin. Longitudinal sections from gastrocnemius (gast) and
diaphragm (diap) muscles at P15 (A-D) and P60 (E-H) revealed no defects in the
mutants. At 6 months, defects were observed in gastrocnemius and diaphragm
muscles in mutants (J,L-N,P), but not in wild types (I,K,O). Pathological
changes included enlarged (J,L, arrows) and bent (M, arrow) muscle fibers, and
centrally located nuclei (L,N,P, arrowheads). Abnormal muscle fiber morphology
was also evident in proximity to MTJs (compare O with P). (Q-X)
Six-month-old mice were injected with Evans Blue Dye (EBD) and analyzed for
dye uptake by bright field illumination of whole-mount tissue. Wild-type
diaphragm (Q) and gastrocnemius muscle (S) did not show dye uptake, unless the
muscle fibers were damaged (U) (damage was occasionally observed in wild-type
and mutant mice). No dye was incorporated in mutant fibers (R,T). (V-X) Dye
uptake was analyzed in sections using immunofluorescence microscopy. Tendon
(t) but not muscle (m) in wild type (V) and mutants (W) had incorporated dye.
In control damaged muscle from wild types (X), dye uptake was observed.
(Y) At 6 months of age, a mild increase in serum creatine kinase levels
was observed in the mutants (n
4; mean ± s.e.). Scale bars:
250 µm in A-P; 200 µm in V-X.
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