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Figure 1


Fig. 1. tiy121 mutants have a cell cycle defect. (A-D) Phosphorylated Histone H3 staining (PHH3, red) marks mitotic cells in wild-type embryos at the shield (A) and 10-somite stage (C). No mitotic nuclei are seen in tiy121 embryos at the shield (B) or 10-somite stage (D). Nuclei are stained with DAPI (blue). (E,F) Wild-type (E) and tiy121-/- (F) embryos at 30 hpf. (G,H) BrdU labeling (red) in the trunks of wild type (G) and tiy121-/- (H) at the 14-somite stage. Asterisks label the notochord. BrdU was injected into the yolk at the 8-somite stage. In C-F, anterior is left; in G and H, anterior is up.





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