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Figure 1


Fig. 1. EKLF expression is initiated prior to erythroid commitment in the mouse fetal liver. (A) Schematic of the Peklf-GFP transgene after site-specific, uni-directional integration into the Ainv18 homing site, yielding the Peklf-GFP ES cell clone. (B-D) Flow cytometric analysis of GFP (B) and CD71/Ter119 expression (C) in fetal liver cells derived from E13.5 Peklf-GFP embryos, as quantified in Table 1. GFP+/Ter119- cells selected for colony assays are marked in D. (E-H) Erythroid (CFU-E and BFU-E), megakaryocyte, granulocyte and macrophage progenitor-derived colonies from GFP+/Ter119- cells were quantified and compared with those derived from GFP-/Ter119- cells. Cells were morphologically assessed by cytospin and May-Grünwald/Giemsa staining. (E) Erythroid cells; (F) megakaryocyte; (G) granulocytes; (H) macrophages. Scale bars: 5 µm. (I) Enrichment is defined as a colony number greater than 10% of total colonies (red vertical line) because ~10% of Ter119- sorted fetal liver cells at E13.5 are GFP+. The arithmetic mean of three independent experiments ±s.d. is shown.





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