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Fig. 2. CheWnt3 is required for the development of axial polarity and oral
fates. (A) Gastrulation and elongation along the oral-aboral axis
in a normal Clytia embryo (top row) was completely blocked in
Wnt3-MO-injected embryos (bottom row), shown fixed at the early gastrula stage
(15 hpf). (B) Complete loss of morphological axis in Wnt3-MO-injected
embryos at late gastrula (20 hpf) stage. Cell contours were visualised by
phalloidin (green) and nuclei by To-Pro3 (red). Gastrulation was severely
delayed compared with uninjected embryos. The exact timing and site of
residual cell ingression formation varied. (C) Equivalent 2-day-old
planulae. No morphological oral-aboral axis is discernable in Wnt3-MO-injected
embryos; however, endoderm formation has recovered. (D) Representative
in situ hybridisation image of characteristic oral CheBra expression
in early gastrulae (15 hpf) that is lost in Wnt3-MO-injected embryos.
(E,F) Loss of CheBra expression in oral ectoderm, and
of CheAxin in oral endoderm and ectoderm in Wnt3-MO-injected planulae (1.5
day). (G,H) Expansion of aboral FoxQ2A expression to
nearly the entire body in Wnt3-MO-injected embryos fixed at early gastrula and
planula stages (1.5 day). (I) Loss of expression of
oral-ectoderm-expressed ligands CheWntX2, CheWnt9,
CheWntX1A and CheWnt5 in Wnt3-MO-injected embryos fixed at
the planula stage (1.5 day). Scale bars: 40 µm.
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