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Fig. 3. Differential expression of Smyhc genes in craniofacial muscles.
(A) Lateral and ventral views of the dorsal group of craniofacial
muscles at 96 hpf with slow troponin C (stnnC), smyhc1 (1 to
249 bp), smyhc2 (1 to 324 bp) or smyhc3 (1 to 129 bp) in
situ hybridisation (see Fig.
1A,D,E,G for gross location of these muscles). The lap and do lack
smyhc1 expression while smyhc3 expression is restricted to
the lap. (B) Ventral views showing ventral craniofacial muscles at 96
hpf with slow troponin C (stnnC), smyhc1 (1 to 249 bp),
smyhc2 (1 to 324 bp) or smyhc3 (1 to 129 bp) in situ
hybridisation. The ima, sh, iob and posterior tv lack smyhc1
expression. (C) Lateral and ventral views of the dorsal group of
craniofacial muscles at 96 hpf showing colocalisation of slow MyHC S58 antigen
with smyhc2 (1 to 324 bp) in situ hybridisation and, in the ao and
lo, with smyhc1:gfp. (D) Ventral view at 96 hpf showing
colocalisation of smyhc1:gfp and smyhc2 (1 to 324 bp) in
situ hybridisation with slow MyHC S58 antigen. In the am, some fibres express
smyhc2 and S58 antigen but not smyhc1:gfp (blue arrows).
Some fibres express S58 antigen but neither smyhc2 nor
smyhc1:gfp (white arrows). The extraocular muscles have zones of S58
antigen-expressing fibres that co-express smyhc1:gfp and zones that
do not (pink arrows). (E) Ventral view at 96 hpf showing expression of
slow troponin C in situ hybridisation (stnnC) in all slow MyHC S58
antigen-expressing fibres. Scale bars: 25 µm. Abbreviations: abh, abductor
hyoideus; am, adductor mandibulae; ao, adductor operculi; do, dilator
operculi; hh, hyohyoidus; ih, interhyoideus; ima, intermandibularis anterior;
imp, intermandibularis posterior; io, inferior oblique; iob, inferior
obliquus; ir, inferior rectus, lap, levator arcus palatini; lo, levatator
operculi; sh, sternohyoideus; tv, transversus ventralis.
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