DEV020560 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. In situ hybridization of Nr5a2 and Cyp11a1. (A,B) An ovary from a 26-day-old Kras^flox/+;Cyp19-Cre mouse showing the absence of Nr5a2 transcripts in abnormal follicle-like structures (arrows). (C,D) An ovary from a 3-month-old Kras^flox/+;Cyp19-Cre mouse showing the absence of Nr5a2 transcripts in abnormal follicle-like structures (arrows). (E,F) An ovary from a 26-day-old wild-type mouse (48 hours after hCG injection) showing the presence of Cyp11a1 transcripts in newly formed corpus lutea (arrows). (G,H) An ovary from a 26-day-old Kras^flox/+;Cyp19-Cre mouse (48 hours after hCG injection) showing the absence of Cyp11a1 transcripts in abnormal follicle-like structures (arrows).

• Supplemental Figure S2 -

  Fig. S2. Phosphorylation of ERK1/2 and AKT in pre-ovulatory ovarian follicles. (A) Phosphorylation of MEK1/2, ERK1/2, PDK1 and AKT was detected in wild-type ovaries at selected time points of eCG/hCG treatment. The amounts of total AKT and β-actin were also detected as loading control. (B) Immunofluorescence of phospho-ERK1/2 in a pre-ovulatory follicle of the wild-type ovary at 2 hours post-hCG. (C) Immunofluorescence of phospho-AKT in a pre-ovulatory follicle of the wild-type ovary at 48 hours post-eCG. (D) Immunofluorescence of phospho-AKT in a pre-ovulatory follicle of the wild-type ovary at 2 hours post-hCG.