spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. KrasG12D conditional knock-in mice develop ovarian lesions with altered granulosa cell proliferation, differentiation and apoptosis. (A) Size differences in wild-type (WT) and LSL-KrasG12D;Cyp19-Cre ovaries at various ages. (B-D) Histology of WT (B) and LSL-KrasG12D;Cyp19-Cre (C,D) ovaries at 6 months of age. NF, normal follicle; AF, abnormal follicle. (E,F) BrdU incorporation assay in 12-week-old WT (E) and LSL-KrasG12D;Cyp19-Cre (F) ovaries. Abnormal follicle-like structures are indicated by arrows (as below). (G,H) Immunofluorescent detection of phospho-histone H3 (pHH3, red) in 12-week-old WT (G) and LSL-KrasG12D;Cyp19-Cre (H) ovaries. (I,J) BrdU incorporation (I) and immunofluorescence for the mitosis marker phospho-histone H3 (J) indicate slightly increased levels of proliferation in KrasG12D-expressing granulosa cells of antral follicles, as compared with wild type. (K,L) Immunohistochemical detection of PCNA in 12-week-old WT (K) and LSL-KrasG12D;Cyp19-Cre (L) ovaries. (M,N) Apoptosis assays in 4-week-old WT (M) and LSL-KrasG12D;Cyp19-Cre (N) ovaries, 2 hours after hCG treatment. (O,P) Immunofluorescent detection of cleaved caspase 3 (CC3) in 12-week-old WT (O) and LSL-KrasG12D;Cyp19-Cre (P) ovaries.





Right arrow Return to article