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Figure 4


Fig. 4. KrasG12D downregulates genes essential for granulosa cell differentiation and ovulation. (A) qRT-PCR of ovulation-related genes from mouse whole ovary mRNAs. Six ovaries from different animals were analyzed. (B) KrasG12D downregulated the expression of Fshr and prevented the FSH-induced expression of Lhcgr in cultured granulosa cells. Expression of KrasG12D was induced by infecting the cells with an adenoviral vector encoding Cre recombinase (Ad-Cre). FSH (100 ng/ml) was added to the medium of cells infected, or not, with Ad-Cre. NT, non-treated. Ppib was amplified by RT-PCR in the same samples, as loading control.





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