DEV016725 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. Wild-type mouse forebrain slice recovered at E12.5. Neural precursor cells were infected with eGFP-expressing retrovirus to mark cells and allow observation of their movement (green). Brain slices were cultured for 48 hours and then visualised by confocal fluorescence time-lapse microscopy for 6 hours. Note the ventricular-to-pial orientation and migratory activity of the wild-type cells. Cells leaving the cell cycle and migrating towards the pial surface are indicated with arrows.

• Movie 2 -

  Movie 2. C3G^gt/gt mutant forebrain slice recovered at E12.5. Neural precursor cells were infected with eGFP-expressing retrovirus to mark cells and allow observation of their movement (green). Brain slices were cultured for 48 hours and then visualised by confocal fluorescence time-lapse microscopy for 6 hours. C3G^gt/gt mutant cells lack orientation. Instead of showing one or two major processes, C3G^gt/gt mutant cells exhibit multiple minor, often branching processes without ventricular-to-pial orientation. Examples are marked with arrows.

• Movie 3 -

  Movie 3. Cortical neuroepithelial explant of a wild-type E10.5 mouse embryo plated on complex extracellular matrix and cultured. Twenty-two hour time-lapse movie taken from day 3 to day 4 of culture using phase-contrast optics. Ample migration of wild-type neurons can be observed.

• Movie 4 -

  Movie 4. Cortical neuroepithelial explant of a C3G^gt/gt mutant E10.5 embryo plated on complex extracellular matrix and cultured. Twenty-two hour time-lapse movie taken from day 3 to day 4 of culture using phase-contrast optics. A complete failure of neuronal migration can be observed in the C3G^gt/gt mutant culture. Nevertheless, the C3G^gt/gt mutant explants contain viable cells and increase in size.