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Fig. 7. C3G^gt/gt mutant cortical neuroepithelial cells fail to extend processes and fail to migrate on complex or defined matrix in vitro. (A-N) Cortical neuroepithelial explants of wild-type (A,C,E,G,I-K) or C3G^gt/gt mutant (B,D,F,H,L-N) E10.5 mouse embryos were plated on complex extracellular matrix (A-D,I-N) or recombinant laminin (E-H) and cultured; (A,B) day 3 of culture, (C-H) day 6, (I-N) day 3 to day 4. For time-lapse, see Movies 3, 4 in the supplementary material. (G,H) Cultures on laminin were stained for the neuronal marker type III β-tubulin (red) and counterstained with bisbenzimide (blue). Differential interference contrast images (A-F), epifluorescence images (G,H) and time-lapse phase-contrast images (I-N). (A,C,E,G,I) Processes and migrating neurons were seen in wild-type (arrowheads) but not in C3G^gt/gt mutant cultures. Dashed line, migration front. Scale bar: 72 µm in A-D; 37 µm in E-H; 70 µm in I-N.

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