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Fig. 3. Aberrant hair follicle development and global expression of placode and
hair shaft markers in activated β-catenin mutant embryos.
(A-J) Histological analysis of dorsal skin from control littermate and
KRT14-Cre Ctnnb1(Ex3)fl/+ mutant embryos reveals the
failure of hair follicle downgrowth in mutants. Mutant skin displays
evaginations (B,D,J; arrows) and fails to stratify normally (H; arrow). (G,H)
Higher magnification views of E and F, respectively. (K-X) The placode
marker Shh (in situ hybridization, brown; K,L) and the dermal
condensate marker alkaline phosphatase (AP; enzymatic assay, purple brown;
M,N) are expressed broadly in the mutant. Expression of the inner root sheath
marker GATA3 (O,P) and the outer root sheath marker SOX9 (Q,R;
immunofluorescence, red) is reduced or absent, but hair shaft keratins (AE13
antibody, immunohistochemistry, brown) are ectopically expressed in mutant
ectoderm (S,T). Proliferation is decreased (U,V; Ki67 immunofluorescence, red)
and the pattern of apoptosis/terminal differentiation is altered (W,X; TUNEL
staining, green) in mutant ectoderm. Arrows indicate expression; dashed lines
mark dermal-epidermal boundaries. Samples were E17.5 (K-N,Q-X) or newborn
(O,P). Scale bars: bar in F applies to A-F; in H, to G,H; in J, to I,J; in X,
to K-X.
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