spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. GFP-PCNA is a single-cell marker of S phase in Fucus serratus zygotes. (A) Eggs were biolistically co-loaded with Texas Red dextran (red images) and GFP-PCNA (green images), and visualized using 2-photon microscopy. Fertilization was confirmed using Calcofluor white (CFW), shown as the white edge in the red images. The same representative zygote is shown 0.5 hours (left) after fertilization (AF) and 3.5 hours after fertilization (right). (B) Nuclear GFP-PCNA/Texas Red dextran ratios (R) were plotted relative to their starting values (R0) for zygotes (white circles) and unfertilized eggs (black circles). Zygotes displayed significant nuclear GFP-PCNA increases between 3 and 5 hours after fertilization. Data are mean±s.e.m.; *P<0.03. (C) The nuclear GFP-PCNA increase seen 4 hours after fertilization (controls are white bars; `Fert', zygotes; `Un', unfertilized eggs) is completely abolished following treatment with the DNA polymerase inhibitor aphidicolin (20 µM; black bars). Data are mean±s.e.m.; *P<0.05.





Right arrow Return to article