DEV015826 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Dpp-insensitive modules are partially buffered in lateral regions of the disc. Confocal images of wing discs (dorsal upwards, anterior leftwards) showing reporter-LlcZ (red) and omb-Gal4>UAS-eGFP expression (green). The schematic depicts module number and organization (green oval represents activator, red symbolizes the SMM complex). Discs were stained in parallel and visualized using identical settings. (A) L12M3 in which the lone SMM site in module 3 was mutated, drives expression throughout the wing pouch. (B) The L13 reporter includes at least four modules and directs a lateral domain of lacZ expression that does not overlap with omb>GFP. (C) L13M3 contains the same lesion in module 3 present in L12M, but expression was not detected in medial regions. However, the lateral domain of expression was slightly wider and now abuts the omb>GFP boundary (compare with L13).

• Supplemental Figure S2 -

  Fig. S2. No additional sites mediating Dpp-dependent repression are present immediately distal to the module-3 SMM site. Wing discs stained for lacZ to visualize expression of a wild-type L10 reporter and L10M3 in which the SMM site in module 3 has been mutated. The schematic illustrates the additional sequences distal to the SMM site (511 bp) in L10, compared with the shorter L12 fragment (97 bp). (A) The L10 reporter is expressed in lateral regions of the disc and is absent from medial regions, in a pattern similar to L12 (see Fig 2A). (B) The L10M3 reporter is expressed throughout the disc, similar to L12M3 (see Fig 3D), indicating that no additional sequences mediating repression are present immediately distal to the SMM site.