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Figure 4


Fig. 4. The brk promoter contains multiple modular regulatory elements. (A-E) Transgenic reporters containing ~780 bp fragments (green bars), centered on SMM sites drive brk-like expression patterns in the wing disc. Red ovals mark the location of 11 predicted SMM sites (#1-11) within the brk promoter at -271, -2165, -3097, -5175, -6627, -7653, -8023, -8174, -8206, -13,833 and -15,983 bp. (A-D) Expression patterns derived from fragments L17, L16, L15 and L14 containing modules 10, 7/8/9, 5 and 4, respectively. Module 4 drives the highest levels of expression laterally. Module 5 drives weak expression (the image was enhanced by increasing exposure time). (E) Module 3 (L12) is shown for comparison.





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