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Figure 5


Fig. 5. Shn interacts with the short-range co-repressor dCtBP. (A) Full-length Shn and ShnCT, a polypeptide sufficient for Dpp-dependent repression of brk, are shown. Zinc-finger domains are marked in blue. A ~100 residue domain required for repression (red bar) includes a CtBP interaction motif PMDLT, which was mutated in ShnCTM as shown. (B) ShnCT interacts with dCtBP but not with Gro. Extracts from S2 cells transfected as indicated, were immunoprecipitated with anti-Myc and probed with anti-Flag. Expression levels were monitored by probing separate blots with anti-Flag or anti-Myc. Wild-type ShnCT bound dCtBP but not Gro, while ShnCTM failed to interact with dCtBP. (C-E) The CtBP interaction motif contributes to repression in vivo. (C) The brkX47 reporter is expressed ubiquitously in shn- embryos. (D) In shn- embryo, Hsp70-Gal4-driven expression of ShnCT restores brk-lacZ repression in the dorsolateral ectoderm and rescues dorsal closure defects. (E) ShnCTM is unable either to repress brk-lacZ or to rescue the shn- morphology.





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