DEV016378 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 -

  Adobe PDF

• Supplemental Figure S1 -

  Fig. S1. Tie2-Cre- and Tie1-Cre-mediated deletion of β1 integrin cause growth retardation and embryonic lethality at midgestation. Fixed control and Tie2- or Tie1-Cre embryo pairs imaged under a dissecting microscope. Arrows indicate pericardial sacs; arrowheads indicate hemorrhages. Scale bars: 200 µm.

• Movie 1 -

  Movie 1. Progressive capillary network formation in control P-Sp explant cultures. Timelapse movie of a control P-Sp explant culture. GFP-positive ECs were visualized by a Tie1-GFP transgene. Note that in this control explant, ECs remain connected to one another and demonstrate progressive capillary network formation. Timelapse images were captured at 15-minute intervals, and the movies display ten frames per second.

• Movie 2 -

  Movie 2. β1 integrins are required for capillary remodeling in P-Sp explants. Timelapse movie of a Tie2-Cre mutant P-Sp explant culture. GFP-positive ECs were visualized by a Tie1-GFP transgene. Note the presence of aberrant EC clusters and the failure to form a capillary network. Extensive cell death, visualized as cells rounding and disintegrating, is frequently observed in mutant explants. Timelapse images were captured at 15-minute intervals, and the movies display ten frames per second.