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Fig. 1. Tie2- and Tie1-Cre mediate efficient deletion of
β1 integrins in mouse embryos. Gene deletion analyses of
Tie2-Cre (A-D) and Tie1-Cre (E,F)
mutants as compared with controls. (A) Genomic PCR analysis of e8.5 embryos
demonstrates recombination (rec.) of β1 integrin in embryos carrying
Tie2-Cre and two floxed alleles of β1. (B) e8.5 cryosections
were stained with anti-CD31 (red), anti-β1 integrin (Ha2/5, green) and
DAPI (blue). (C) Collagenase-dissociated e8.5 embryonic cells were plated onto
fibronectin and stained with anti-β1 (HMβ1-1, green), anti-β3
integrin (red) and DAPI (blue). Endothelial cell (EC) identity in C was
determined by co-staining with anti-CD31 (not shown). (D) Focal contacts in
isolated ECs. Bars are means + s.e.m. of two (β1) or three (β3)
experiments. **P<0.01 by one sample t-test and
*P<0.05 by Student's t-test. (E) Genomic PCR
analysis of e10.5 embryos from Tie1-Cre matings. (F) e9.5
cryosections were stained with anti-CD31 (red), anti-β1 integrin (green)
and DAPI (blue). Arrows, primary head veins; arrowheads, β1
integrin-negative endothelium; ys, yolk sac blood islands; a-da, anterior
dorsal aortae; p-da, posterior dorsal aortae; nt, neural tube; g, gut; ve,
visceral endoderm. Also see Fig.
3F for diagram of embryonic structures. Scale bars: 20 µm.
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