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Fig. 5. EC-autonomous role for β1 integrins in angiogenic
remodeling. (A-D) Still images of control (A,C) and
Tie2-Cre mutant (B,D) mouse embryonic P-Sp explant cultures at the
indicated timepoints. GFP-positive ECs were visualized by a Tie1-GFP
transgene. Aberrant EC clusters are evident in Tie2-Cre mutant
explants, but not in control explants. The OP9 feeder cells, not visible in
the fluorescence images, were a confluent monolayer on top of which the ECs
grew out from the P-Sp. The dashed lines indicate the approximate location of
the P-Sp explants. See also Movies 1, 2 in the supplementary material.
(E-H) Capillary morphogenesis of embryonic
Itgb1flox/flox ECs immortalized with polyoma middle T
antigen and subsequently infected with adenovirus (Ad), in the absence of any
antibodies (E,F) or in the presence of anti-
v integrin plus
anti-β3 (G) or anti-β1 (H) integrin function-blocking antibodies.
Phase-contrast images were captured after 4 hours in culture. Scale bars: 100
µm.
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