spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Jaws-/- digits develop ectopic joints. (A) Longitudinal cavities lacking Alcian Blue staining form in Jaws-/- digits (E18.5). Higher-magnification views of digit 3 are shown to the right of each limb, with arrows indicating joint position and orientation. The arrowhead indicates failed knee joint formation proximal to a lack of tibial ossification in the Jaws-/- hindlimb. (B) Whole-mount in situ hybridization for Gdf5 expression (E14.5). (C) Histological and in situ hybridization analysis showing longitudinal expression of joint interzone markers in individual Jaws-/- hindlimb digits (E14.5). (D) Exclusion of Bmpr1b RNA and collagen 2 protein expression from ectopic Jaws-/- joints (E14.5). (E) Ectopic joints express Gdf5 but not markers of hypertrophic chondrocytes (Col10a1), osteoblasts (Runx2), or tendons and ligaments (Scx) (E18.5 forelimb digits). AB/vK, Alcian Blue/von Kossa. (F) Defective cavitation of E18.5 ectopic joint (arrow), evidenced by the absence of CD44 immunostaining (arrowhead, wild-type staining).





Right arrow Return to article