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Fig. 5. Amnioserosa extension through rapid cell elongation without
intercalation. (A) Three-dimensional projections. DE-Cad-GFP. AS
cell elongation. Four marked neighboring cells retain contacts. (B)
Initial AS cell elongation rates. Blue lines indicate average elongation rate
for five cells, DE-Cad-GFP embryos. Black lines indicate average elongation
rate for five cells, Moesin ABD-GFP embryos. Red line indicates the all embryo
average. (C) DE-Cadherin-GFP level quantification through confocal
stacks. Background-corrected AP and DV contact values normalized to DV contact
value for each cell. Five cell averages per embryo are shown (four embryos,
different colors). Squares, DV contacts; circles, AP contacts (arrows).
(D) Three-dimensional projections. DV contacts (white arrows),
DE-Cad-GFP looping (yellow arrows). Scale bars: 5 µm.
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