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Figure 1


Fig. 1. hsd-1 expression in the XXX cells globally regulates DAF-16 activity. (A-C) hsd-1 is exclusively expressed in the paired XXX cells of the head. (A) Phsd-1::GFP expression. (B) Psdf-9::RFP expression in the XXX cells. (C) Merged image showing that Phsd-1::GFP co-localizes with Psdf-9::RFP. (D-F) Level of DAF-16::GFP nuclear localization. Anterior of animal indicated by the arrows. (D) Diffuse (cytoplasmic) GFP in wild-type control L2 larva. Partially diffuse and punctate (nuclear) GFP (E) and almost entirely punctate GFP (F) in hsd-1(mg433); ncr-1(nr2022) L2d larvae.





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