DEV017905 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Hindbrain patterning and proliferation in cN1ICD embryos is normal. (A) Transverse section through a E9.5 cN1ICD mutant showing GFP expression in the ectopic lateral placode (bracket). (B) Dorsal view of cN1ICD embryos showing expression of hindbrain markers. r3, rhombomere 3; r4, rhombomere 4; r5, rhombomere 5; r5/6, rhombomeres 5 and 6. (C) Pax2 (red) only partially overlaps with the N1ICD (GFP, green) domain in cN1ICD mutants. (D) Cell proliferation in Pax2-Cre; GFP reporter and cN1ICD: GFP embryos. Error bars indicate s.e.m. Scale bars: 100 µm.

• Supplemental Figure S2 -

  Fig. S2. Comparison of Notch pathway gene expression in Notch1 and Rbpjκ CKO mutants. (A) Dorsal views showing normal expression of hindbrain markers in Notch1 mutants. (B) Jag1 and Hes1 expression is reduced in Notch1 mutants. (C) Jag1 is expressed normally in cRbpjκ mutants but Hes1 is significantly reduced. The otic placode still forms in these mutants. Arrow indicates Hes1-expressing cells. Insets in B and C show corresponding whole-mount images. Brackets/dotted outline show the otic region. Scale bars: 50 µm.

• Supplemental Figure S3 -

  Fig. S3. Wnt reporter domain length and otic cup length in cN1ICD embryos. The Wnt reporter domain length and otic cup length is expanded in cN1ICD embryos at E8.75-E9.25. Brackets indicate n placode values. *P<0.005, Student’s t-test. Error bars indicate s.e.m.