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Fig. 4. SRC64B and DRL and their mammalian orthologs physically associate.
(A) Drosophila Kc cells were transfected with the indicated
expression constructs, lysates were immunoprecipitated (IP) with antibodies
specific to DRL (anti-HA) or SRC64B (anti-MYC) and then immunoblotted (WB)
with the reciprocal antibody to detect co-immunoprecipitation. The expression
of DRL and SRC64B was confirmed by immunoblotting of the whole-cell extract
(WCE). DRL and SRC64B specifically co-immunoprecipitate in the presence or
absence of WNT5 protein. (B) The mammalian orthologs of DRL and SRC64B,
RYK and c-SRC, physically interact as assayed by their co-immunoprecipitation
from transfected human 293T cell lysates. The expression of RYK-HA and
untagged c-SRC was confirmed by WCE immunoblot. RYK derived from
c-SRC-overexpressing cells migrates faster than control RYK species,
presumably owing to altered post-translational processing. Endogenous c-SRC
protein is visible in lanes 1 and 2 of the lowermost blot.
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