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Fig. 6. Serial transplantation of the CNH. (A) Serial grafting
experiments between stage 15 HH and stage 20 HH embryos. (B-D) Embryos
immediately after grafting (left) and after incubation (right). (B) CNH
from a stage 15 HH GFP+ embryo grafted to a host embryo and
incubated for 20 hours. (C) CNH region from the first host was grafted
to a stage 15 HH host and incubated for 20 hours. (D) CNH region from
the second host was grafted to a stage 15 HH host and incubated for 48 hours.
(E) Transverse section of the third host embryo immunostained for the
neuronal marker TUJ1 (red), which labels some GFP+ cells (arrow).
Blue, nuclear stain. Scale bars: B-D, 0.5 mm; E, 0.1 mm.