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Fig. 4. wg and ss are functionally linked in maxillary/antennal programs. (A) In wild-type L3/pupal transition, Wg (green) and Ss (red), but not Dll (blue), proteins are detected in maxillary (mx) cells. (B) In wild type, Dll is first detected in the mx primordium of white pre-pupae. (C,D) Both wg and ss induce a maxillary-to-antennal transformation through a mechanism involving reciprocal activation. (C) In wg^gof L3 larvae (ptc-GAL4>UAS-wg^ts shifted to 18°C at 5 days AEL), Ss (red) is precociously induced on the band of Wg-expressing cells (green) within the neo-antennal territory (mx>a). (D) In ss^gof (ptc-GAL4>UAS-ss) L3 larvae, the mx>a transformation is associated with precocious Wg expression (green) overlying the band of Ss-expressing cells (red) in the mx field. (E,F) In ss^- mutant pupae, normal expression is observed for Wg (green) or Dll (red) proteins. (G) Clone of pygo^- cells (no green GFP marker, outlined) where nuclear Ss protein (red) is cell-autonomously absent from mutant maxillary cells. (H) Clone of Dll^- cells [no green GFP marker (outlined) and no Dll (blue)] where nuclear Ss protein (red) is present in mutant maxillary cells. (E-H) These genotypes lead to reduction or loss of adult Mx palps (mx^-). Antennal discs are oriented with posterior towards the right and dorsal towards the bottom; a and mx indicate antenna and maxillary primordia, respectively.

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