DEV019125 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Bilateral grafts of QT6-Shh cells in the presumptive BA1 territories. (A) Bilateral grafts of QT6-Shh cells (arrows) at the level of the mesencephalic-rhombencephalic boundary at 7 ss. (B-F) E10 operated embryo showing the normal beak and two supernumerary lower beaks on the right sides (B,C) and a complex supplementary structure on the left side (E,F). (D) Front view of the same embryo showing normal upper and lower beak (in the middle) and induced (left and right) lower jaws.

• Supplemental Figure S2 -

  Fig. S2. Graft of control QT6 cells in the presumptive BA1 territory gives rise to normal morphology and gene expression. (A,B) Right (grafted) and left (control) sides of the head at E10 showing a normal lower beak. (C) Skeleton of the right jaw evidenced by using Alcian Blue staining for cartilages and Alizarin Red for bones. (D-F) Whole-mount in situ hybridization for Bmp4, Pitx1 and Shh show normal expression patterns on both sides (n=4).

• Supplemental Figure S3 -

  Fig. S3. Consecutive diagonal cross sections of the mandibular bud (Md) in situ hybridized for Shh, Fgf8, Bmp4 and Pitx1. (A) Facial view of E4 chicken embryo with diagonal lines representing the proximodistal sections (C-F). (B) Right lateral view of the same embryo shows the duplicated BA1 (I-I′). (C-F) Sections from the lateral to the medial part of the Md show variations of expression for each gene. Fgf8 presents a high expression in the most lateral sections (C3) and decreases in the medial ones (F3). Conversely, Bmp4 is almost absent in the most lateral sections (C2) but high in more medial ones (D2-F2).