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Figure 2


Fig. 2. Localization, at E4, of QT6-Shh cells grafted in BA1. (A) Facial view showing the oral cavity and facial buds of an embryo grafted with QT6-Shh cells on its right side (left on the figure). The experimental mandibular bud (Md) is hyperplasic and presents an outgrowth in its lateroposterior region (arrow). (B) Lateral right view of the same embryo showing the Md and maxillary bud (Mx) on the operated side and the positions of serial, distal-to-proximal, sections (C-E). (C,C') Distal section showing no quail cells with the QCPN mAb. This part of the grafted Md presents a lateroposterior outgrowth (arrow in C'). (D,D') Medial section: few QCPN+ quail cells are present in this Md region (arrowheads in D'). (E-E'') Proximal sections: most of the Shh+ (E'') and QCPN+ (E,E') cells are found in the proximal region of BA1 (arrowheads) close to the boundary with BA2. Some cells are detected in BA2 proximal region (arrow). Scale bar: 100 µm in C-E''.





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