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Figure 3


Fig. 3. β-catenin activity is required early for cell survival. (A,C) Transverse sections of an E10.5 control embryo (A) and a conditional β-catenin loss-of-function mutant (C) stained with X-gal to show the distribution of lineage-labeled cells. (B,D) Alternate sections assayed for cell survival by TUNEL (brown, arrows); nuclei are counterstained with methyl green. (D) Note the significant increase in TUNEL+ cells in the HoxB6Cre; R26R; β-catlof mutants. (A-D) Images of forelimb (FL) level sections taken at the same magnification.





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