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Figure 4


Fig. 4. Ventral dermal progenitors in the flank mesenchyme express En1 and contribute extensively to ventral dermal cells. (A-D) In En1Cre; R26R embryos, En1 lineage-marked (blue) cells are found in the flank mesenchyme at E10.5 (A,B) and in the midline of the ventrum by E11.5 (C,D). (A,C) Whole mounts; (B,D) sections. Insets (B,D) are lower magnification views of the sections. (E) En1 lineaged-marked cells are found extensively in the epidermis and dermis of E16.5 En1Cre; R26R embryos. (F) En1Cre-ER; R26R embryos given Tamoxifen at E10.5; at E16.5 β-gal+ cells are present only in the dermis (arrows). Black dashed line demarcates the epidermis from the dermis.





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