DEV017038 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Defective invasion of embryonic blood vessels into Lkb1^lox/−, Mox2-Cre placenta. Hematoxylin and Eosin-stained sections of E9.5 wild-type (A) and Lkb1^lox/−, Mox2-Cre (B) placentas, demonstrating fewer embryonic vessels in the mutant. Embryonic vessels are defined by the presence of nucleated red blood cells and are outlined in yellow. Asterisk, maternal blood lacuna; gc, giant trophoblast cell layer; la, labyrinthine layer.

• Supplemental Figure S2 -

  Fig. S2. Unaltered EC proliferation and apoptosis rates in Lkb1^lox/−, Tie1-Cre yolk sacs. (A,B) Ki-67 staining of wild-type (A) and Lkb1^lox/−, Tie1-Cre (B) yolk sacs at E11.5. (C,D) Percentage of Ki-67 positive ECs in wild-type (C) and Lkb1^lox/−, Tie1-Cre (D) yolk sacs. Bars indicate mean±s.d. of three embryos. (E,F) TUNEL assay of E11.5 wild-type (E) and Lkb1^lox/−, Tie1-Cre (F) yolk sacs. Insets show TUNEL-positive cells related to normal tissue modelling around the notochord as a positive staining control. e, endoderm; EC, endothelial cell; mes, mesenchymal cell.