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Fig. 7. Deletion of L1-binding domains from TAG-1 disrupts responses to Sema3A and ventral spinal cord. (A) E13.5 TAG-1^a/a DRG co-cultured with Sema3A-expressing Cos7 cells. (B) P/D ratio of number of axons from TAG-1^a/a DRG cultured with Sema3A (mean=0.677, s.e.m.=0.068, n=8) compared with wild type, TAG-1-null and L1-null results (from Fig. 4E). (C) E13.5 TAG-1^a/a DRG co-cultured with VSC. (D) P/D ratio of number of axons from TAG-1^a/a DRG cultured with ventral spinal cord (mean=0.68, s.e.m.=0.042, n=15) compared as in B. ^***P<0.001, ^**P<0.01; unpaired t-test. Scale bar: 100 µm.

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