|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. dmd-3 is likely to be a direct target of TRA-1A and is
sufficient to trigger hermaphrodite tail tip retraction. (A) DIC
images of an adult wild type X0 male (i), an adult tra-1(e1099) XX
pseudomale (ii), an adult dmd-3(ok1327) X0 male (iii) and an adult
tra-1(e1099); dmd-3(ok1327) XX pseudomale (iv). Overlaid
dmd-3::YFP/DIC images of a wild-type late mid-L4 X0 male (v) and a
tra-1(e1099) late mid-L4 XX pseudomale (vi) expressing
dmd-3::YFP (fsIs2). (B) Sequences of the TRA-1A
consensus binding site (Zarkower and
Hodgkin, 1993; Yi et al.,
2000), the putative TRA-1A binding site in the dmd-3
promoter and the 
TRA-1 mutation. The single nucleotide difference
between the consensus site and the dmd-3 site is shown in green;
nucleotides mutated in TRA-1 are shown in red. (C)
DMD-3::GFP/DIC (i, ii, iv, v, vii, viii, xi, xii),
DMD-3::GFP/MH27 antibody staining (red) (ix, xiii) and DIC
(iii, vi, x, xiv) images of late L3 (i, iv, vii and xi), mid-L4 (ii, v, viii,
ix, xii, and xiii) and adult (iii, vi, x, xiv) males (i-vi) and hermaphrodites
(vii-xiv) carrying E(ht)::DMD-3::GFP
(fsIs9) (i-iii and vii-x) or
E(ht)TRA-1::DMD-3::GFP
(fsIs7) (iv-vi and xi-xiv). Expression of
E(ht)TRA-1::DMD-3::GFP in
hermaphrodite tail tip hypodermal cells (numbers in xii) and hindgut (hg)
(white arrowheads in xi and xii) is indicated. Gray arrowheads (vii and viii)
indicate expression in phasmid neurons (ph). Solid red arrowheads (ix and
xiii) mark intact hyp8-11 boundaries; the dashed red arrowhead (xiii)
indicates cell fusion. White arrows (ii, iv, v, xi and xiii) mark hyp8-11
retraction.
|
