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Fig. 5. EFF-1 is regulated by dmd-3 and mab-3. (A)
AJM-1::GFP (syIs78)/DIC and DIC images of wild-type and
eff-1(ok1021) L4 (i,ii) and adult (iii,iv) males. The broken green
arrowhead indicates cell fusion; the solid green arrowhead marks intact cell
boundaries. The white arrows indicate tail tip retraction. (B) Tail tip
expression of an EFF-1::GFP translational reporter
(fsEx135) in male (i-v) and hermaphrodite (vi) larvae. (C)
Expression of EFF-1::GFP in wild type (i), mab-3
(ii), dmd-3 (iii,iv) and dmd-3; mab-3 (v) mid-L4 males. The
examples in iii and iv represent the range of EFF-1::GFP
expression levels seen in dmd-3 mutants. (D) Categorization of
EFF-1::GFP fluorescence intensity in wildtype (n=39),
mab-3 (n=24), dmd-3 (n=61) and mab-3;
dmd-3 (n=33) mid-L4 males and wild-type mid-L4 hermaphrodites
(n=31) based on confocal images. (E) Expression of
EFF-1::GFP::outron::mCherry in a wild-type
mid-L4 male (i-iii) and hermaphrodite (iv-vi). GFP (i,iv), mCherry (ii,v) and
GFP/mCherry (iii,vi) overlays are shown.
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