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Figure 4


Fig. 4. Effect of trx RNAi on association of trxG and PcG proteins with their target genes. Salivary gland polytene chromosomes were prepared from wild-type third instar Drosophila larvae and from the line expressing trx RNAi as described (Petruk et al., 2006). The overall structure of these chromosomes is indistinguishable (Petruk et al., 2006). (A) Trx is essential for association of Ash1 and Asx. Binding of Trx (green), Ash1 (red, column 2) and Asx (red, column 3) is completely abolished in the trx RNAi line, whereas binding of the control protein, Ecdysone receptor (EcR, green) is unaffected (column 1). (B) Trx is not essential for association of the trxG BRM complex and Kis. Components of the BRM complex, Brm and Osa, and trxG protein Kis (red) bind to their sites on polytene chromosomes in a line expressing trx RNAi. Trx binding is in green. (C) Association of the PcG complexes PRC1 and PRC2 is not affected by induction of trx RNAi. The intensities and the number of bands for the components of PRC1, Pc and Ph, and the component of the PRC2, E(z) (all in red), are not significantly affected in the trx RNAi line.





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