DEV013482 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. (A-A′) Optical sections of Tg(AANAT2:GFP) epiphysis at 2 days of development stained with a triple red opsin, exorhodopsin, rhodopsin probe and GFP. All opsin-positive cells are Tg(AANAT2:GFP)+; conversely, only 87.2±4.14% of Tg(AANAT2:GFP)+ cells are positive for one of the three opsins. (B-C′) Optical sections of Tg(HuC:GFP) (B,B′) and Tg(AANAT2:GFP) (C,C′) epiphyses at 48 hours labelled with a Pax6 antibody and GFP. Tg(HuC:GFP)+ cells are also positive for Pax6 (blue arrowheads). Numerous Pax6+, Tg(HuC:GFP)− neuroepithelial cells are observed in the ventrolateral part of the epiphysial area (white arrowheads). Pax6 and Tg(AANAT2:GFP) expression is mostly exclusive except for rare cells expressing both markers (blue arrowheads).

• Supplemental Figure S2 -

  Fig. S2. (A-F) Confocal sections of Tg(AANAT2:GFP) (A-C) and Tg(HuC:GFP) (D-F) epiphysis stained with a caspase 3 antibody and GFP in wild-type (A), mib mutant (B) and DAPT-treated embryos (C) at 48 hours of development. The number of apoptotic cells is increased in mib mutant compared with wild-type embryos and in DAPT-treated embryos compared with mib mutants. (G) Counts of caspase 3+ cells, Tg(HuC:GFP)+/caspase 3+ and Tg(AANAT2:GFP)+/caspase 3+ cells. Numbers indicate average±s.d. *P<0.05; **P<0.001; ***P<0.0005 using a t-test. nd, not determined.