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Fig. 6. The photoreceptors and the projection neurons are born simultaneously
and Notch activity is required in cycling progenitors. (A-D')
Co-labeling of BrdU (red) with either Tg(HuC:GFP) (A,C) or Tg(AANAT2:GFP)
(B,D) in 48-hour-old embryos that have been subjected to a 2-hour BrdU-pulse
starting at 18 hours (A-B') or 20.5 hours (C-D'). White arrowheads
indicate double-labeled cells. (E) Proportion of BrdU+;GFP+ cells over
total number of GFP+ cells after a 2-hour pulse of BrdU. Embryos transgenic
for Tg(HuC:GFP) or Tg(AANAT2:GFP) were subjected to a pulse of BrdU starting
from various stages. The percent of BrdU+ cells was evaluated at 48 hours.
(F-H) Average numbers of Islet1+ neurons, Tg(AANAT2:GFP)+ and
Tg(HuC:GFP)+ cells in the epiphysis of DMSO and DAPT-treated embryos. The
x-axis indicates the stage at which the treatment starts. Anterior is
upwards. Scale bars: 16 µm. Error bars represent s.d.
*P<0.05; **P<0.001;
***P<0.0005 using a t-test.
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