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Figure 6


Fig. 6. Nodal induces elevated noggin expression in left LPM. (A-D) Ventral views of lacZ staining in 5s Nog-/- (A,C) and Chrd-/-;Nog-/- (DKO) (B,D) mouse embryos. C and D are high magnification views of the boxed regions from A and B, respectively, showing the left and right LPM regions. (E-H) Cultured embryos at 5s hybridized with Lefty2 (E,F) or Nog (G,H) probe. Embryos were cultured with DMSO (E,G) or with the Nodal signaling inhibitor SB-431542 in DMSO (F,H). White lines surround left LPM in G and H. (I,J) Cultured 5s embryos hybridized with Lefty2 (purple) and GFP (red) probes. (I) Control embryo with GFP vector introduced into right LPM. (J) Embryo with GFP and Nodal vectors introduced into right LPM. Red arrows indicate expression of GFP in LPM, arrowheads that of Lefty2. (K,L) Cultured 5s embryos hybridized with Nog (purple) and GFP (red) probes. (K) Control embryo with GFP vector introduced into right LPM. (L) Embryo with GFP and Nodal vectors introduced into right LPM. Gray line surrounds right LPM. Red arrows indicate expression of GFP in LPM, arrowheads that of Nog.





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